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Immunohistochemical staining of nerve grafts.

By Xin Yu (124814), Yanfang Jiang (162530), Lu Lu (392), Xu Gong (176615), Xiguang Sun (176618), Zhaopeng Xuan (176620) and Laijin Lu (176623)

Abstract

<p>(A) Immunohistochemical staining of collected nerve grafts from sham control and xenotransplanted mice, were immunostained using anti-IFN-γ, anti-IL-17, and anti-IL-4 antibodies with DAB as the chromogen with a Hematoxylin counterstain. Both antibodies demonstrated scattered immunoreactivity in the infiltrating mononuclear cells. Representative images are shown. Magnification: 200×. (B) IFN-γ+ cells and IL-17+ cells increased in xenotransplanted group from day 6 to day 30 compared to the sham controls. There is no significant difference of IL-4+ cells between the recipients and control group. *, p<0.05; **, p<0.01; *** p<0.001. (We performed experiment twice, and stained 2 sections for IFN-γ and IL-17 respectively of each animal.)</p

Topics: Neuroscience, Immunology, staining
Year: 2012
DOI identifier: 10.1371/journal.pone.0034419.g002
OAI identifier: oai:figshare.com:article/330996
Provided by: FigShare
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