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Effect of anti-miR-148a on cell phenotype.

By Ke Yuan (172453), Zhaorui Lian (172457), Bill Sun (172462), Marcia M. Clayton (172469), Irene O. L. Ng (172473) and Mark A. Feitelson (50820)

Abstract

<p>HepG2CAT, HepG2X and HepG2URG11 cells were (A) transiently transfected with anti-miR-148a or (B) stably transduced with recombinant lentivirus encoding anti-miR-148a. In both cases, cell growth was evaluated by MTT assay on days 1, 2 and 3 of culture. All values were normalized to cells treated only with transfection reagent and are presented as a ratio of test:control O.D. values. (C) Serum starved cells were analyzed by flow cytometry on day 3 after the addition of serum. The percentage of cells in G1,S,G2/M phases are indicated in each panel.</p

Topics: Genetics, Chemistry, Cancer, Infectious Diseases, Virology, anti-mir-148a
Year: 2012
DOI identifier: 10.1371/journal.pone.0035331.g003
OAI identifier: oai:figshare.com:article/327175
Provided by: FigShare
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