<i>Ih</i> gene is expressed in dopaminergic neurons.

Abstract

<p>(A) Confocal projection of adult brain showing dopaminergic neurons labeled with GFP using the Tyrosine Hydroxilase Gal4 driver (<i>THG4;UAS:GFP</i>). D, dorsal, L, lateral, OE, oesophagus, OL, optic lobe. (B) RT-PCR amplification of <i>Ih</i> (112 bp) and <i>ple</i> (133 bp) RNAs from 200 isolated dopaminergic and non-dopaminergic neurons sorted by Fluorescence Activated Cell Sorting (FACS). Image shows amplification of <i>Ih</i> RNA in dopaminergic, <i>ple</i>-expressing neurons. Failure to PCR-amplify the neuronal <i>ple</i> RNA isoform in non-dopaminergic neurons (GFP negative cells) confirms that cells have been correctly sorted. Asterisks in lanes 1, 2, and 5 point to nonspecific bands, which were obtained in the negative controls (<i>DmIh</i> mutant brain-RNA extract for <i>Ih</i> –lane 1- and GFP-negative cell-RNA extract for <i>ple</i> –lane 5-).</p

Download PDF:
_{Sorry, we are unable to provide the full text but you may find it at the following location(s):}

https://figshare.com/articles/... (external link)