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<i>Ih</i> gene is expressed in dopaminergic neurons.

By Alicia Gonzalo-Gomez (166343), Enrique Turiegano (166351), Yolanda León (166359), Isabel Molina (166372), Laura Torroja (166381) and Inmaculada Canal (166386)

Abstract

<p>(A) Confocal projection of adult brain showing dopaminergic neurons labeled with GFP using the Tyrosine Hydroxilase Gal4 driver (<i>THG4;UAS:GFP</i>). D, dorsal, L, lateral, OE, oesophagus, OL, optic lobe. (B) RT-PCR amplification of <i>Ih</i> (112 bp) and <i>ple</i> (133 bp) RNAs from 200 isolated dopaminergic and non-dopaminergic neurons sorted by Fluorescence Activated Cell Sorting (FACS). Image shows amplification of <i>Ih</i> RNA in dopaminergic, <i>ple</i>-expressing neurons. Failure to PCR-amplify the neuronal <i>ple</i> RNA isoform in non-dopaminergic neurons (GFP negative cells) confirms that cells have been correctly sorted. Asterisks in lanes 1, 2, and 5 point to nonspecific bands, which were obtained in the negative controls (<i>DmIh</i> mutant brain-RNA extract for <i>Ih</i> –lane 1- and GFP-negative cell-RNA extract for <i>ple</i> –lane 5-).</p

Topics: Biochemistry, Neuroscience, Physiology, dopaminergic
Year: 2012
DOI identifier: 10.1371/journal.pone.0036477.g003
OAI identifier: oai:figshare.com:article/312461
Provided by: FigShare
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