Expression and purification of recombinant NA in mammalian cells.

Abstract

<p>Human TB-NA (Hokkaido) fused to the Tetrabrachion stalk (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037779#pone.0037779.s001" target="_blank">Fig. 1</a>B) was cloned into the pApex-3 vector and expressed in HEK293T cells. Media, flow-through, and purified NA were loaded onto SDS-PAGE and detected by anti-FLAG WB (left panel) or stained by Coomassie (right panel). Secreted FLAG-reactive NA was detected by anti-FLAG WB. Based on the anti-FLAG WB the column flow-through did not contain any residual FLAG reactive NA.</p

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