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Expression and purification of recombinant NA in mammalian cells.

By Peter M. Schmidt (159712), Lindsay G. Sparrow (159719), Rebecca M. Attwood (159728), Xiaowen Xiao (159735), Tim E. Adams (159746) and Jennifer L. McKimm-Breschkin (159753)

Abstract

<p>Human TB-NA (Hokkaido) fused to the Tetrabrachion stalk (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037779#pone.0037779.s001" target="_blank">Fig. 1</a>B) was cloned into the pApex-3 vector and expressed in HEK293T cells. Media, flow-through, and purified NA were loaded onto SDS-PAGE and detected by anti-FLAG WB (left panel) or stained by Coomassie (right panel). Secreted FLAG-reactive NA was detected by anti-FLAG WB. Based on the anti-FLAG WB the column flow-through did not contain any residual FLAG reactive NA.</p

Topics: Biochemistry, Biotechnology, purification, recombinant, na, mammalian
Year: 2012
DOI identifier: 10.1371/journal.pone.0037779.g004
OAI identifier: oai:figshare.com:article/298185
Provided by: FigShare
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