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mRNA expression in wild type and LRRK2 KO BMDM’s following LPS stimulation.

By Nicolas Dzamko (157468), Francisco Inesta-Vaquera (157472), Jiazhen Zhang (157474), Chengsong Xie (157477), Huaibin Cai (157480), Simon Arthur (157483), Li Tan (157486), Hwanguen Choi (157490), Nathanael Gray (157494), Philip Cohen (157498), Patrick Pedrioli (157501), Kristopher Clark (88841) and Dario R. Alessi (157506)

Abstract

<p>Bone marrow derived macrophages from LRRK2 knockout mice and wild type littermate controls were plated at a density of 1×10<sup>6</sup> in 2 ml of tissue culture media. Cells were treated with 100 ng/ml LPS 24 h after plating and RNA collected at the indicated time points. Gene expression was normalized to GAPDH and the fold increase in mRNA expression compared to wild type macrophages at time 0 was calculated by the comparative Ct method (2<sup>−ddct</sup>). Data is presented as mean fold increase in expression ± S.E.M. Experiments were performed with n = 4 in duplicate.</p

Topics: Biochemistry, Molecular Biology, Immunology, lrrk2, ko, lps
Year: 2012
DOI identifier: 10.1371/journal.pone.0039132.t003
OAI identifier: oai:figshare.com:article/294603
Provided by: FigShare
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