Genetic requirements for <i>lacO</i>-dependent Chk1 phosphorylation.

Abstract

<p>* indicates phospho Chk1. A. The phosphorylation status of Chk1 was detected with α-HA over 24 hours following induction of Rad3-GFP/LN in <i>lacO</i> positive cells in the genetic background indicated. Increased time is indicated by the wedge. B. Equivalent experiments assessing Chk1 phosphorylation in <i>rad</i>⁺<i>lacO</i> positive cells upon induction of the indicated constructs. C. Chk1 phosphorylation was assessed upon expression of Rad3-GFP/LN in the indicated genetic backgrounds at t = 22 hours. D. The phosphorylation status of Chk1 was assessed following induction of Rad3-GFP/LN in <i>lacO</i> positive cells in <i>rad</i>⁺ and the indicated double mutant where H2A cannot be phosphorylated. E. The indicated constructs were expressed in <i>rad</i>⁺ or <i>rad9-T412</i> cells and Chk1 phosphorylation monitored. Rad9 T412 phosphorylation is required for the interaction of 9-1-1 complex with Rad4^TopBP1.</p

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