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Genetic requirements for <i>lacO</i>-dependent Chk1 phosphorylation.

By Su-Jiun Lin (155081), Christopher P. Wardlaw (155086), Takashi Morishita (155091), Izumi Miyabe (155097), Charly Chahwan (155106), Thomas Caspari (155113), Ulrike Schmidt (61417), Antony M. Carr (155119) and Valerie Garcia (155125)

Abstract

<p>* indicates phospho Chk1. A. The phosphorylation status of Chk1 was detected with α-HA over 24 hours following induction of Rad3-GFP/LN in <i>lacO</i> positive cells in the genetic background indicated. Increased time is indicated by the wedge. B. Equivalent experiments assessing Chk1 phosphorylation in <i>rad</i><sup>+</sup><i>lacO</i> positive cells upon induction of the indicated constructs. C. Chk1 phosphorylation was assessed upon expression of Rad3-GFP/LN in the indicated genetic backgrounds at t = 22 hours. D. The phosphorylation status of Chk1 was assessed following induction of Rad3-GFP/LN in <i>lacO</i> positive cells in <i>rad</i><sup>+</sup> and the indicated double mutant where H2A cannot be phosphorylated. E. The indicated constructs were expressed in <i>rad</i><sup>+</sup> or <i>rad9-T412</i> cells and Chk1 phosphorylation monitored. Rad9 T412 phosphorylation is required for the interaction of 9-1-1 complex with Rad4<sup>TopBP1</sup>.</p

Topics: Biological Sciences, requirements, chk1
Year: 2012
DOI identifier: 10.1371/journal.pgen.1002801.g005
OAI identifier: oai:figshare.com:article/287347
Provided by: FigShare
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