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<i>EVER1</i> and <i>EVER2</i> are expressed in lymphocytes.

By Maciej Lazarczyk (155392), Cécile Dalard (155397), Myriam Hayder (155401), Loïc Dupre (155407), Béatrice Pignolet (155412), Slawomir Majewski (155416), Francoise Vuillier (155420), Michel Favre (155262) and Roland S. Liblau (155426)

Abstract

<p><b>A.</b> Expression of <i>EVER1</i> and <i>EVER2</i> genes in different mouse organs was assessed by qRT-PCR. Expression in the spleen was set as 100%. For each organ at least 3 independent experiments were performed. <b>B.</b> Expression of <i>EVER1</i> and <i>EVER2</i> genes in murine T and B cells was determined by qRT-PCR. At least 3 independent experiments were performed for each cell type. Expression of EVER1 protein in murine splenocytes (<b>C</b>) and purified T cells (<b>D</b>) was determined by western blot. An EVER1-specific antibody (Abcam; ab67326) was used. A single band at ≈100 kDa was detected (predicted EVER1 MW = 91 kDa). <b>E.</b> 293 T cells were transfected with plasmids encoding either EVER1-FLAG or EVER2-FLAG fusion protein. The fusion protein was immunoprecipitated with anti-FLAG antibody and subsequently a western-blot was performed using the anti-FLAG antibody or two different anti-EVER1 antibodies (Ab n<sup>o</sup>1 - Abcam; ab67326; Ab n°2 - Osenses; OSR00223W).</p

Topics: Microbiology, Immunology, Developmental Biology, Virology, immunology, Virology, microbiology, dermatology
Year: 2012
DOI identifier: 10.1371/journal.pone.0039995.g001
OAI identifier: oai:figshare.com:article/288055
Provided by: FigShare
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