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Combination of NFκB and PI3-kinase/AKT inhibitors induce synergistic inhibition of cell viability and induction of apoptosis in PEL cell lines.

By Azhar R. Hussain (154380), Saeeda O. Ahmed (154383), Maqbool Ahmed (154386), Omar S. Khan (154391), Sally Al AbdulMohsen (154394), Leonidas C. Platanias (154396), Khawla S. Al-Kuraya (154399) and Shahab Uddin (154400)

Abstract

<p>(<b>A</b>) <b>Combination of sub-toxic doses of Bay11-7085 and LY294002 suppresses growth of PEL cells.</b> BC1 and BC3 cells were treated with either 1 µM Bay11-7085 or 5 µM LY294002 alone or in combination for 24 hours. Cell viability was measured by MTT assays as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039945#s2" target="_blank">Materials and Methods</a>. The graph displays the mean +/− SD (standard deviation) of three independent experiments, * p<0.05, statistically significant (Students <i>t</i>-test). (<b>B and C</b>) <b>Combination of Bay11-7085 and LY294002 induces efficient apoptosis in PEL cells.</b> BC1 and BC3 cells were treated with either 1 µM Bay11-7085 or 5 µM LY294002 alone or in combination for 24 hours. Following treatment, cells were stained with FITC conjugated annexin V/PI and cells were analyzed by flow cytometry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039945#s2" target="_blank">material and methods</a> (<b>B</b>). Bar graph denotes percentage apoptosis of three independent experiments (<b>C</b>). (<b>D</b>) <b>Combination of sub-toxic doses of Bay11-7085 and LY294002 induced apoptosis is caspase dependent.</b> BC1 cells were treated with either 1 µM Bay11-7085 or 5 µM LY294002 alone or in combination for 24 hours. Following treatment, cells were lysed and equal amounts of proteins were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted with antibodies against caspase-9, caspase-3, cleaved caspase-3 and PARP. Beta-actin was used for equal loading.</p

Topics: Biochemistry, Cell Biology, Molecular Biology, Cancer, Hematology, inhibitors, induce, synergistic, inhibition, viability, induction, apoptosis, pel
Year: 2012
DOI identifier: 10.1371/journal.pone.0039945.g006
OAI identifier: oai:figshare.com:article/286966
Provided by: FigShare
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