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Developmentally regulated expression of testosterone-responsive miRNAs.

By Subbarayalu Panneerdoss (110522), Yao-Fu Chang (110521), Kalyan C. Buddavarapu (110524), Hung-I Harry Chen (110520), Gunapala Shetty (150365), Huizhen Wang (150370), Yidong Chen (11610), T. Rajendra Kumar (150378) and Manjeet K. Rao (110547)

Abstract

<p>(<b>A</b>) Diagram of the mouse X chromosome showing location of two miRNA clusters. Cluster 1 includes six miRNAs (panels b–g) and cluster 2 includes two miRNAs (panels h and i). (<b>B</b>) Real-time RT-PCR analysis of selected miRNA (panels a–h) expression in RNA isolated from purified Sertoli cells from mice of ages shown. Sertoli cells were pooled from seven mice for 7 days (<i>n = </i>2 different experiments) and from six mice for each of 13 days (<i>n = </i>2 different experiments), 21 days (<i>n = </i>3 different experiments), and 60 days (<i>n = </i>3 different experiments). All values were normalized against 5S levels. MiRNAs in cluster 2 exhibit quantitative as well as temporal collinear expression pattern.</p

Topics: Molecular Biology, Physiology, regulated, testosterone-responsive
Year: 2012
DOI identifier: 10.1371/journal.pone.0041146.g003
OAI identifier: oai:figshare.com:article/276466
Provided by: FigShare
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