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HIV p24Gag-specific T cell cytokine responses in spleens of uninfected or <i>Mtb</i>-infected mice vaccinated with MultiHIV DNA/protein.

By Lech Ignatowicz (145016), Jolanta Mazurek (145013), Chaniya Leepiyasakulchai (148597), Markus Sköld (148601), Jorma Hinkula (2323), Gunilla Källenius (2292337) and Andrzej Pawlowski (145021)

Abstract

<p>Splenocytes from <i>Mtb</i>-infected or uninfected mice, which were immunized and boosted with MultiHIV DNA/protein, were isolated 4 wk post-vaccination, restimulated <i>ex vivo</i> with p24Gag antigen, and stained for CD4 and CD8 markers and for intracellular IFN-γ, TNF, and IL-2 (details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041205#s4" target="_blank">Materials and Methods</a>). Numbers of p24Gag-specific CD4+ (A) and CD8+ (B) T cells producing one of three cytokines were determined by flow cytometry. Mean number of single cytokine-producing T cells ± SEM are shown (6–8 animals/group from one individual experiment; **: P<0.01). Flow cytometry plots depict representative intracellular cytokine staining of CD4+ (C) and CD8+ (D) T cells. The cytokine profile of p24Gag-specific T cells was determined in two separate experiments.</p

Topics: Microbiology, Immunology, Infectious Diseases, p24gag-specific, cytokine, responses, spleens, uninfected, mice, vaccinated, multihiv
Year: 2012
DOI identifier: 10.1371/journal.pone.0041205.g005
OAI identifier: oai:figshare.com:article/275660
Provided by: FigShare
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