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Sch B inhibits TGF-β induced EMT of 4T1 and primary human breast cancer cells <i>in vitro</i>.

By Zhen Liu (74646), Biao Zhang (148194), Kun Liu (148201), Zonghui Ding (74645) and Xun Hu (74649)

Abstract

<p>Cells were treated with Sch B (5 µg/ml) and TGF-β (5 ng/ml) as described in Materials and Methods. (A) Representative photos of TGF-β induced morphological change of 4T1 cells with or without pretreatment of Sch B. (B) Fluorescence immunostaining of cellular E-cadherin, vimentin, fibronectin, and F-actin. (C) Western blotting of E-cadherin, vimentin, Snail, Slug, ZEB1, with β-actin as the internal control. (D) and (E), TGF-β induced migration and invasion of 4T1 cells with or without pretreatment of Sch B. (F) Representative photos of TGF-induced the morphological change of primary breast cancer cells with or without pretreatment of Sch B and western blotting of E-cadherin and vimentin of primary breast cancer cells from three individual patients.</p

Topics: Cancer, inhibits, induced, emt, 4t1, cancer, cells
Year: 2012
DOI identifier: 10.1371/journal.pone.0040480.g007
OAI identifier: oai:figshare.com:article/273335
Provided by: FigShare
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