DDX4 during androgen manipulation.

Abstract

<p><b>A.</b> Immunohistochemical localisation of DDX4 (green) in control testis. Staining is observed in late pachytene spermatocyte (PSC) cytoplasm and chromatoid body precursor structures in the perinuclear region (arrowheads). <i>Inset</i> shows control for the primary antibody. <b>B.</b> During androgen blockade (TE+Flutamide), DDX4 immunostaining intensity increased in late pachytene spermatocyte (PSC) cytoplasm. In panels A and B, cell nuclei were visualized with TOPRO (red). <b>C.</b> Evaluation of androgen-responsive pI isoforms of DDX4; the upper panel shows a representative image for the 2D-Western during androgen blockade (-Androgen, TE+Flutamide) compared to androgen replacement with T24 (TE+T24, +Androgen). Fourteen distinct pI isoforms were resolved. Results of the densitometric analysis of pooled samples (lower panel) from –Androgen and +Androgen groups (<i>for details see </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041718#pone-0041718-g003" target="_blank"><i>Figure 3</i></a><i> legend</i>) revealed that one isoform showed a significant (p<0.05, t-test) difference between these groups (asterix), however the other isoforms showed trends to increase or decrease with androgen replacement. Data is shown as mean ± SD (n = 3 separate experiments).</p

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