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<i>Pseudomonas putida</i> Response to Cadmium: Changes in Membrane and Cytosolic Proteomes

By Anna Manara (2058784), Giovanni DalCorso (2058781), Cecilia Baliardini (2058775), Silvia Farinati (2058778), Daniela Cecconi (186831) and Antonella Furini (2058772)

Abstract

<i>Pseudomonas putida</i> is a saprophytic bacterium with remarkable environmental adaptability and the capacity to tolerate high concentrations of heavy metals. The strain <i>P. putida-Cd001</i> was isolated from soil contaminated with Cd, Zn and Pb. Membrane-associated and cytosolic proteomes were analyzed to identify proteins whose expression was modulated in response to 250 μM CdSO<sub>4</sub>. We identified 44 protein spots in the membrane and 21 in the cytosolic fraction differentially expressed in Cd-treated samples compared to untreated controls. Outer membrane porins from the OprD and OprI families were less abundant in bacteria exposed to Cd, whereas those from the OprF and OprL, OprH and OprB families were more abundant, reflecting the increased need to acquire energy sources, the need to maintain membrane integrity and the process of adaptation. Components of the efflux system, such as the CzcB subunit of the CBA system, were also induced by Cd. Analysis of the cytosolic proteome revealed that proteins involved in protein synthesis, degradation and folding were induced along with enzymes that combat oxidative stress, showing that the entire bacterial proteome is modulated by heavy metal exposure. This analysis provides new insights into the adaptation mechanisms used by <i>P. putida-Cd001</i> to survive in Cd-polluted environments

Topics: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Ecology, Inorganic Chemistry, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, saprophytic bacterium, cytosolic proteome, CBA system, 44 protein spots, cytosolic fraction differentially, efflux system, energy sources, cytosolic proteomes, 250 μ M CdSO 4., metal exposure, Pseudomonas putida Response, combat oxidative stress, adaptation mechanisms, CzcB subunit, OprB families, membrane integrity, protein synthesis, strain P, Cytosolic ProteomesPseudomonas putida, Outer membrane porins, OprI families, analysis
Year: 2012
DOI identifier: 10.1021/pr300281f.s002
OAI identifier: oai:figshare.com:article/2499925
Provided by: FigShare
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