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BRAFV600E is required and sufficient to suppress AKT phosphorylation in a subset of melanoma cells.

By Brenden Chen (144880), Christine Tardell (144883), Brian Higgins (144884), Kathryn Packman (93334), John F. Boylan (144887) and Huifeng Niu (144889)

Abstract

<p>(A) Western blot analysis of AKT phosphorylation 24 hours after BRAF or CRAF knock-down in A375 melanoma cell line 4 hour post RG7167 treatment. (B) Western blot analysis of AKT substrates (FOXO1, GSK3α/β, PRAS40) phosphorylation 24 hours after BRAF knock-down in A375 melanoma cell line. (C) Western blot analysis of AKT phosphorylation 24 hours after BRAF knock-down in LOX melanoma cell line. (D) Western blot analysis of AKT, MEK, and ERK phosphorylation in CHL1 melanoma cell line 24 hours after transient transfection (wild-type BRAF or BRAFV600E) and treated with RG7167 for 4 hours.</p

Topics: Cancer, suppress, akt, phosphorylation, subset, melanoma
Year: 2012
DOI identifier: 10.1371/journal.pone.0042598.g003
OAI identifier: oai:figshare.com:article/267001
Provided by: FigShare
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