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Tripelennamine sensitizes autophagy-deficient yeast mutants.

By Ulrich Schlecht (145060), Robert P. St. Onge (145063), Thomas Walther (145066), Jean-Marie François (145070) and Ronald W. Davis (12925)

Abstract

<p>(A) Heatmap and dendrogram depicting hierarchical clustering of homozygous deletion pool data from three independent experiments. Quantile normalized and log2-transformed microarray fluorescence signals were analyzed using the Significance Analysis of Microarrays (SAM) software (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042853#s4" target="_blank">Methods</a>) and identified 49 genes (indicated on the right-hand side) that were significantly depleted in the presence of tripelennamine (TA) compared to a “no drug” control. Darker shades of red indicate higher fluorescence signals and therefore a higher abundance of that strain in the pool (see legend). (B) Growth of the <i>ho</i>Δ<i>/HO</i> (control) and <i>neo1</i>Δ<i>/NEO1</i> heterozygous deletion strains (see legend) were determined in the presence of various concentrations of (TA) (indicated on the x-axis). Growth rates relative to the “no drug” control (calculated as a ratio of AvgG, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042853#s4" target="_blank">Methods</a>) were determined for each concentration and are plotted on the y-axis. (C) Sporulation efficiency of the <i>ho</i>Δ<i>/HO</i> control strain (black curve) and the <i>neo1</i>Δ<i>/NEO1</i> heterozygous deletion strain (red curve) sporulated in the presence or absence of TA is indicated as percent spores on the y-axis. Both strains were incubated in sporulation media for 48 hours at various concentrations of TA (indicated on the x-axis) and percentage of spores was determined by microscopy. A total of 100 cells were counted for every condition.</p

Topics: Biochemistry, Microbiology, Cell Biology, Genetics, sensitizes, autophagy-deficient, yeast
Year: 2012
DOI identifier: 10.1371/journal.pone.0042853.g004
OAI identifier: oai:figshare.com:article/266238
Provided by: FigShare
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