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Comparison of ITS versus COI DNA barcodes in species–level identification.

By Rowena F. Stern (122915), Robert A. Andersen (142310), Ian Jameson (142314), Frithjof C. Küpper (142316), Mary-Alice Coffroth (142319), Daniel Vaulot (142322), Florence Le Gall (142325), Benoît Véron (142328), Jerry J. Brand (142330), Hayley Skelton (142332), Fumai Kasai (142334), Emily L. Lilly (142336) and Patrick J. Keeling (82468)

Abstract

<p>Panel A and B refer to ITS and COI respectively. Dark grey shading indicates intraspecific distances whereas light grey interspecific distances. Y axis shows percentage of named species and genera that fall into pairwise distance categories (X-axis). Both A and B share same X-axis. Although both ITS and CO1 barcodes fall within the 0–0.02% range, note how ITS has a sizeable gap in genetic distance within species compared to between species, that is lacking for COI marker. In this study 2% or less PWD between strains was used as a species cut off, which encompassed 94% of strains. Abbreviations: Sym: <i>Symbiodinium</i> and Sym gp. A? refers to unknown group A <i>Symbiodinium</i> sp.; Karl.: <i>Karlodinium</i>; K. ven.: <i>Karlodinium veneficum</i>; C.sp.: <i>Cryptoperidiniopsis</i> sp.; Scr.: <i>Scrippsiella</i>; S.troch.: <i>Scrippsiella trochoidea</i>.</p

Topics: Biophysics, Biochemistry, Microbiology, Genetics, Inorganic Chemistry, coi, dna, barcodes
Year: 2012
DOI identifier: 10.1371/journal.pone.0042780.g002
OAI identifier: oai:figshare.com:article/261366
Provided by: FigShare
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