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Cids-2 downregulates the expression of PGL-1 in <i>C. elegans</i> somatic cells.

By Tomoyasu Sugiyama (141024), Rie Sugioka-Sugiyama (141027), Kazumasa Hada (141029) and Ryusuke Niwa (141031)

Abstract

<p>(A) Differential interference contrast (DIC, left raw) and fluorescent (right raw) images of <i>C. elegans</i> (<i>bnIs1</i>[<i>pie-1p::gfp::pgl-1</i>]) fed <i>E. coli</i>. that carry a plasmid for control, <i>cids-2</i>, or <i>lin-35</i> RNAi. GFP signals in the head regions (white brackets) were elevated in <i>cids-2</i> and <i>lin-35</i> RNAi worms. (B) The bar graph shows the calculated signal intensity of control, <i>cids-2</i>, or <i>lin-35</i> RNAi (mean ± S.D., arbitrary units). The GFP signals of <i>cids-2</i> and <i>lin-35</i> RNAi animals were significantly increased compared to control RNAi worms. *<i>p</i><0.001, compared to control RNAi. (C) Knockdown of <i>cids-2</i> led to the accumulation of the authentic <i>pgl-1</i> transcript. (left) A schema of <i>pgl-1</i> RT-PCR analysis. Synchronized <i>glp-4(bn2)</i> mutant worms at L1 stage were fed <i>E. coli</i>. that carried a plasmid for control, <i>cids-2</i>, or <i>lin-35</i> RNAi and were grown to the L4 stage at the restrictive temperature (25°C). RNAs were then prepared from these L4 animals and subjected to RT-PCR. (right) RT-PCR analysis of the endogenous <i>pgl-1</i> mRNA was performed with three independent samples for each of the RNAi-treated groups, and <i>act-1</i> was used as a control. RT (-), no reverse transcription.</p

Topics: Microbiology, Cell Biology, Molecular Biology, downregulates, pgl-1, somatic
Year: 2012
DOI identifier: 10.1371/journal.pone.0042962.g008
OAI identifier: oai:figshare.com:article/261338
Provided by: FigShare
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