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Up-regulation of <i>Stmn1</i> in human breast cancer tissues.

By Rui Wang (52434), Hong-Bin Wang (128886), Chan Juan Hao (128887), Yi Cui (128888), Xiao-Chen Han (128890), Yi Hu (58993), Fei-Feng Li (128893), Hong-Fei Xia (128895) and Xu Ma (45624)


<p>We pooled two patients' cancer tissues as a sample. (A) The protein level of STMN1 in human breast cancer tissues was detected by western blot. The bands were analyzed using the Quantity One analyzing system (Bio-Rad Laboratory inc.). The expression of β-ACTIN served as an internal control. The black histograms represent the expression level of STMN1 in breast cancer tissues of all (A1), ER/PR−,HER2−(A2), ER/PR+, HER2+ (A3), ER/PR+, HER2− (A4) and ER/PR−,HER2+(A5) and expressed as STMN1 intensity/β-ACTIN intensity to normalize for gel loading and transfer. N represents non-tumor tissues, and T represents tumor tissues. (B) The mRNA level of <i>Stmn1</i> in human breast cancer tissues and adjacent normal breast tissues was detected by qRT-PCR. The expression level of <i>Stmn1</i> was obviously increased in 83.33% individual sample. Statistical analyses were performed to analyze the overall trend of <i>Stmn1</i> in human breast cancer tissues of all (B1), ER/PR−,HER2− (B2), ER/PR+, HER2+ (B3), ER/PR+, HER2− (B4) and ER/PR−, HER2+ (B5). β-actin serves as an internal reference. The <i>y</i>-axis displays the relative log2 ratio of <i>Stmn1</i> normalized to β-actin. *<i>P</i><0.05, **<i>P</i><0.01.</p

Topics: Cell Biology, Molecular Biology, Chemistry, Developmental Biology, Cancer, cancer
Year: 2012
DOI identifier: 10.1371/journal.pone.0046173.g009
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Provided by: FigShare
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