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Functionality of exogenous CD46 in MP<sub>30</sub>CD46-transduced CHO cells. (A), CD46 as complement C3 regulator.

By Gaëlle Gonzalez (110076), Cyrielle Vituret (110077), Attilio Di Pietro (110079), Marc Chanson (110081), Pierre Boulanger (27531) and Saw-See Hong (110083)

Abstract

<p>CHO cells were harvested at 48 h posttransfer, and the CD46-induced protection against complement C3-mediated cell apoptosis was assayed by the percentage of Annexin V-positive cells determined by flow cytometry at increasing doses of complement C3. <b>(B), Kinetics of the gain of adenoviral receptor function by MP<sub>30</sub>CD46-transduced CHO cells.</b> CHO cells were harvested at different times after MP<sub>30</sub>CD46-transfer, and cell permissiveness to the HAdV5F35-GFP vector was assessed by infection with HAdV5F35-GFP at MOI 500. Cells were analyzed for GFP signal at 48 h postinfection. The degree of permissiveness to the vector was expressed as the percentage of GFP-positive cells (left <i>y</i>-axis), and the relative transduction efficiency (RTE; right <i>y</i>-axis). The RTE, in arbitrary units (AU), was given using the formula = (percentage of GFP-positive cells) x (MFI; mean fluorescence intensity).</p

Topics: Biochemistry, Cell Biology, Genetics, exogenous, cd46, cho, complement, c3
Year: 2012
DOI identifier: 10.1371/journal.pone.0052326.g004
OAI identifier: oai:figshare.com:article/191853
Provided by: FigShare
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