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The presence of nifedipine in culture medium inhibits differentiation of ES cell-derived CMs.

By Filomain Nguemo (107007), Bernd K. Fleischmann (107008), Manoj K. Gupta (107010), Tomo Šarić (107014), Daniela Malan (107018), Huamin Liang (30409), Kurt Pfannkuche (30408), Wilhelm Bloch (45980), Heribert Schunkert (44257), Jürgen Hescheler (30413) and Michael Reppel (107026)

Abstract

<p>(<b><i>A</i></b>) Protocol used to determine the effect of nifedipine application during cell differentiation. Note that for beating EBs and single cell electrophysiology and Ca2+ imaging experiments, analysis was performed at least 24 hours after washout to reflect long-term changes instead of acute drugs effects. (<b><i>B–C</i></b>) Representative experiments showing EBs with GFP expressing areas cultured under control conditions (<i>B</i>) and after nifedipine-treatment (10 µM) (<i>C</i>) of ES cells. (<b><i>D–F</i></b>) Representative EBs showing EGFP+ CMs differentiated of ES cells under control conditions (<b><i>D</i></b>), after nifedipine- (<b><i>E</i></b>) and BayK8644- (<b><i>F</i></b>) treatment. (<b>G</b>) Representative FACS analysis of ES cell-derived CMs generated under control, nifedipine- and BayK8644-treated conditions. (Scale bars 50 µm (B, C) and 20 µm (D–F).</p

Topics: Cell Biology, Molecular Biology, Developmental Biology, nifedipine, inhibits, differentiation, es, cell-derived
Year: 2013
DOI identifier: 10.1371/journal.pone.0053407.g001
OAI identifier: oai:figshare.com:article/182880
Provided by: FigShare
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