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Characterizations of action potentials in NRVF infected with Ad-Kir2.1, Ad-Na<sub>V</sub>1.5 and Ad-Cx43.

By Luqia Hou (279399), Bin Hu (122268) and José Jalife (279400)

Abstract

<p><b>A.</b> Action potentials were recorded from triple infected (K/Na/Cx43, 5/20/10 MOI) NRVF (left) but not in K/Cx43 (5/10 MOI) infected NRVF (right). <b>B.</b> Resting membrane potentials in uninfected, K/Na/Cx43 (5/20/10 MOI) infected and K/Cx43 (5/10 MOI) infected NRVF. <b>C.</b> APD<sub>80</sub>, APD<sub>50</sub> and APD<sub>30</sub> measured at different pacing cycle length. <b>D.</b> Representative action potential traces shows that resting membrane potentials and APDs are sensitive to BaCl<sub>2</sub> (1 mM) but not 4-AP (1 mM). <b>E.</b> Resting membrane potentials measured before and after BaCl<sub>2</sub> (1 mM) perfusion, and after washout in K/Na/Cx43 NRVF. <b>F.</b> Quantification of APD<sub>30</sub>, APD<sub>50</sub> and APD<sub>80</sub> before and after 4-AP perfusion. <b>G.</b> Quantification of APD<sub>30</sub>, APD<sub>50</sub> and APD<sub>80</sub> before and after BaCl<sub>2</sub> perfusion. *: p<0.05.</p

Topics: Biochemistry, Molecular Biology, Neuroscience, Physiology, potentials, nrvf, infected
Year: 2013
DOI identifier: 10.1371/journal.pone.0055400.g004
OAI identifier: oai:figshare.com:article/165374
Provided by: FigShare
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