Hydrolysis of L-DNA1 by an L-Hammerhead Ribozyme and D-DNA1 by a D-Hammerhead Ribozyme at various Mg⁺⁺ Concentrations.

Abstract

<p>Left panel: Target L-DNA1 in buffer without MgCl₂ (C), the same with 25 mM of MgCl₂ (C’), Target L-DNA1 with a hammerhead Spiegelzyme at 1, 5, 10 and 25 mM MgCl₂, LL control: L-RNA1 incubated with L-hammerhead. Right panel: Target D-DNA1 in buffer without (C’’) and with 25 mM MgCl₂ (C’’’). Target D-DNA1 and hammerhead ribozyme at 1, 5, 10 and 25 mM MgCl₂, DD control: D- RNA1 incubated with D-hammerhead ribozyme. Arrow identifies hydrolysis site as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054741#pone-0054741-g001" target="_blank">Fig. 1B</a>.</p

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