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Hydrolysis of L-DNA1 by an L-Hammerhead Ribozyme and D-DNA1 by a D-Hammerhead Ribozyme at various Mg<sup>++</sup> Concentrations.

By Eliza Wyszko (285999), Maciej Szymański (55069), Heinz Zeichhardt (286000), Florian Müller (286001), Jan Barciszewski (55070) and Volker A. Erdmann (189926)

Abstract

<p>Left panel: Target L-DNA1 in buffer without MgCl<sub>2</sub> (C), the same with 25 mM of MgCl<sub>2</sub> (C’), Target L-DNA1 with a hammerhead Spiegelzyme at 1, 5, 10 and 25 mM MgCl<sub>2</sub>, LL control: L-RNA1 incubated with L-hammerhead. Right panel: Target D-DNA1 in buffer without (C’’) and with 25 mM MgCl<sub>2</sub> (C’’’). Target D-DNA1 and hammerhead ribozyme at 1, 5, 10 and 25 mM MgCl<sub>2</sub>, DD control: D- RNA1 incubated with D-hammerhead ribozyme. Arrow identifies hydrolysis site as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054741#pone-0054741-g001" target="_blank">Fig. 1B</a>.</p

Topics: Biochemistry, Physics, Chemistry, l-dna1, l-hammerhead, ribozyme, d-dna1, d-hammerhead
Year: 2013
DOI identifier: 10.1371/journal.pone.0054741.g002
OAI identifier: oai:figshare.com:article/168509
Provided by: FigShare
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