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Glutathione determination by ELISA in HeLa and CHP-212.

By Alejandra Idan Valencia-Cruz (286040), Laura I. Uribe-Figueroa (286041), Rodrigo Galindo-Murillo (286042), Karol Baca-López (286043), Anllely G. Gutiérrez (286044), Adriana Vázquez-Aguirre (286046), Lena Ruiz-Azuara (286048), Enrique Hernández-Lemus (286050) and Carmen Mejía (286052)

Abstract

<p>GSH intracellular index was measured by accumulated MCB fluorescence in a microtiter plate reader at 380/460 nm. GSH in non-treated cells was considered as a normalizing top value (100%); whereas negative control cells were MCB plus Cell Lysis Buffer. Data represent means standard deviation of three wells and experiments were replicated once. Differences between groups were assessed (marked with an asterisk) by ANOVA analysis with significance at P0.05.</p

Topics: Cell Biology, Genetics, Biological Sciences, Cancer, elisa, hela
Year: 2013
DOI identifier: 10.1371/journal.pone.0054664.g012
OAI identifier: oai:figshare.com:article/170104
Provided by: FigShare
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