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Virions harvested from siRNA treated cells are efficiently depleted of the siRNA-targeted protein.

By Camille Stegen (290704), Yordanka Yakova (290706), Daniel Henaff (251598), Julien Nadjar (173796), Johanne Duron (290707) and Roger Lippé (290708)

Abstract

<p>143B cells were transfected with Lipofectamine only or siRNAs against the control VP16 or the positive hits Arf1, DDX3X and eIF4H. They were then infected at a MOI of 5 with HSV-1 K26GFP and the supernatants collected at 24 hours. Concentrated supernatants corresponding to equal amounts of VP5 were analyzed and 20 µg of Lipofectamine-treated cell lysates were used as control. The reduced levels of <b>A</b>) VP16, <b>B</b>) ARF1, <b>C</b>) DDX3X and <b>D</b>) eIF4H in newly produced virions in the supernatants were confirmed by Western blotting.</p

Topics: Biochemistry, Microbiology, Genetics, Infectious Diseases, Virology, harvested, sirna, treated, cells, efficiently, depleted, sirna-targeted
Year: 2013
DOI identifier: 10.1371/journal.pone.0053276.g006
OAI identifier: oai:figshare.com:article/184296
Provided by: FigShare
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