CD11c proximal promoter contains binding sites for PU.1 and IRF-4, which are essential for GM-CSF or FLT3L-induced CD11c activation.

Abstract

<p><b>A</b>) Diagram demonstrating the human 5.3 kb CD11c proximal promoter. Transcription factors binding to this region according to ChIP-seq analysis of existing data are illustrated in the diagram. <b>B</b>) ChIP assays on cultured mouse bone marrow cells with GM-CSF for the binding of Pu.1 and Irf-4 to the CD11c proximal promoter region. <b>C</b>) Quantitative PCR on reverse transcribed mRNA from mouse bone marrow cells cultured with GM-CSF or FLT3L to evaluate expression of Pu.1 and Irf-4. Bone marrow cells from mice bearing both CD11c-Cre and Rosa26 loxP-STOP-loxP were transduced with retrovirus carrying either scrambled control shRNA or shRNA against Pu.1 followed by puromycin selection of transduced cells. Immunoblotting <b>D</b>) and Flow cytometric analysis of the transduced cells for the expression YFP in both CD11c⁺ and CD11c⁻ cells 11 days after culturing with GM-CSF <b>E</b>), or FLT3L F).</p