Article thumbnail

CD11c proximal promoter contains binding sites for PU.1 and IRF-4, which are essential for GM-CSF or FLT3L-induced CD11c activation.

By Xue-Jun Zhu (292833), Zhong-Fa Yang (292835), Yaoyu Chen (160290), Junling Wang (252266) and Alan G. Rosmarin (292837)

Abstract

<p><b>A</b>) Diagram demonstrating the human 5.3 kb CD11c proximal promoter. Transcription factors binding to this region according to ChIP-seq analysis of existing data are illustrated in the diagram. <b>B</b>) ChIP assays on cultured mouse bone marrow cells with GM-CSF for the binding of Pu.1 and Irf-4 to the CD11c proximal promoter region. <b>C</b>) Quantitative PCR on reverse transcribed mRNA from mouse bone marrow cells cultured with GM-CSF or FLT3L to evaluate expression of Pu.1 and Irf-4. Bone marrow cells from mice bearing both CD11c-Cre and Rosa26 loxP-STOP-loxP were transduced with retrovirus carrying either scrambled control shRNA or shRNA against Pu.1 followed by puromycin selection of transduced cells. Immunoblotting <b>D</b>) and Flow cytometric analysis of the transduced cells for the expression YFP in both CD11c<sup>+</sup> and CD11c<sup>−</sup> cells 11 days after culturing with GM-CSF <b>E</b>), or FLT3L F).</p

Topics: Cell Biology, Physiology, Immunology, Developmental Biology, proximal, promoter, contains, binding, sites, gm-csf, flt3l-induced, cd11c
Year: 2013
DOI identifier: 10.1371/journal.pone.0052141.g007
OAI identifier: oai:figshare.com:article/191930
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.