Abstract

<p>A) Raft sections containing HPV-16, -18 or -58 genomes were individually probed with MoAb16E4<sub>35–42</sub>, R18E4<sub>53–60</sub> and R58E4<sub>23–30</sub> antibodies (red) and were counterstained with DAPI (blue). The different antibodies allowed type-specific detection of E4 and showed no cross-reactivity amongst the types tested. B) E4 protein expression was detected in HPV-16, -18 and -58 rafts after pre-treatment with solution D, pH 9.0 and autoclaved for 2 min, prior to incubation with MoAb16E4<sub>35–42</sub>, R18E4<sub>53–60</sub> and R58E4<sub>23–30</sub> antibodies (red - upper panels). In the lower panels, sections were pre-treated in the same way prior to incubation with cross-reacting TVG405 or RE4 (green). All sections were counterstained with 4′,6′-diamino-2-diamino-2-phenylindole (DAPI, blue).</p

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Last time updated on 16/03/2018

This paper was published in FigShare.

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