Persistence of <i>L. pneumophila</i> in two-species biofilms formed by <i>K. pneumoniae</i> and <i>Flavobacterium</i> sp. or <i>K. pneumoniae</i> and <i>P. aeruginosa</i>.

Abstract

<p>A base biofilm of either <i>K. pneumoniae</i> strain DMDS 92-08-28a (□) and <i>Flavobacterium sp</i>. strain CDC-65 (◊) (A) or <i>K. pneumoniae</i> strain DMDS 92-08-28a (□) and <i>P. aeruginosa</i> ATCC strain 7700 (◊) (B) pre-formed on stainless steel coupons was inoculated with <i>L. pneumophila</i> strain 130b (▪) on day 3 and flow of 1∶100 R2A began at 1–2 ml/min. Each data point represents the averages and standard deviations of colony counts from a single rod, and the experiments shown here are representative of two experiments.</p