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Identification of the Osx binding site in the promoter of <i>MMP13</i> gene.

By Chi Zhang (9857), Wanjin Tang (298107) and Yang Li (7082)


<p>(A) Deletion analysis of the <i>MMP13</i> promoter-reporter constructs. MMP13-1 kb, MMP13-540 bp, MMP13-210 bp and MMP13-80 bp promoter-reporter plasmids (300 ng each) were cotransfected with 400 ng of the Osx expression plasmid in HEK293 cells. Twenty-four hours post-transfection, cell extracts were prepared and analyzed for luciferase activity and normalized to β-galactosidase activity. (B) The GC-rich element in MMP13-80 is responsible for <i>MMP13</i> promoter reporter activation by Osx. The promoter mutant MMP13-80-M was transfected into HEK293 cells and analyzed as described in panel A. Luciferase activity was normalized by β-galactosidase activity.</p

Topics: Biochemistry, Genetics, Molecular Biology, Physiology, Ecology, Biological Sciences, Developmental Biology, osx, binding, promoter
Year: 2013
DOI identifier: 10.1371/journal.pone.0050525.g005
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Provided by: FigShare
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