Article thumbnail

Identification of the Osx binding site in the promoter of <i>MMP13</i> gene.

By Chi Zhang (9857), Wanjin Tang (298107) and Yang Li (7082)

Abstract

<p>(A) Deletion analysis of the <i>MMP13</i> promoter-reporter constructs. MMP13-1 kb, MMP13-540 bp, MMP13-210 bp and MMP13-80 bp promoter-reporter plasmids (300 ng each) were cotransfected with 400 ng of the Osx expression plasmid in HEK293 cells. Twenty-four hours post-transfection, cell extracts were prepared and analyzed for luciferase activity and normalized to β-galactosidase activity. (B) The GC-rich element in MMP13-80 is responsible for <i>MMP13</i> promoter reporter activation by Osx. The promoter mutant MMP13-80-M was transfected into HEK293 cells and analyzed as described in panel A. Luciferase activity was normalized by β-galactosidase activity.</p

Topics: Biochemistry, Genetics, Molecular Biology, Physiology, Ecology, Biological Sciences, Developmental Biology, osx, binding, promoter
Year: 2013
DOI identifier: 10.1371/journal.pone.0050525.g005
OAI identifier: oai:figshare.com:article/210168
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.