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Percentage of cells stained γ-H2AX positive after irradiation at 8.75 Gy.

By Seog-Young Kim (297940), Juong G. Rhee (297941), Xinxin Song (297942), Edward V. Prochownik (163628), Douglas R. Spitz (10597) and Yong J. Lee (297943)

Abstract

<p>Kinetics of γ-H2AX foci removal after irradiation. Non-stem and CSC-like MDA-MB-435 and MDA-MB-231 cells were irradiated at 8.75 Gy. Various times (0.5–12 hr) after irradiation, cells were fixed and immunostained with anti-phospho-H2AX antibody. Nuclei containing at least six fluorescent foci were considered positive and percentage of cells stained γ-H2AX positive was determined. Error bars represent standard error from the mean for three separate experiments.</p

Topics: Biophysics, Biochemistry, Genetics, Molecular Biology, Developmental Biology, Cancer, cells, stained, irradiation
Year: 2013
DOI identifier: 10.1371/journal.pone.0050423.t002
OAI identifier: oai:figshare.com:article/210470
Provided by: FigShare
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