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Expression of the <i>bioG</i> and <i>bioK</i> genes in <i>E. coli</i> replaces <i>bioH</i> function <i>in vivo</i>.

By Madelyn M. Shapiro (299456), Vandana Chakravartty (299457) and John E. Cronan (299459)

Abstract

<p><i>E. coli</i> strain STL24 (<i>ΔbioH</i>) was transformed with derivative of pBAD322 carrying various <i>bioG</i> or <i>bioK</i> genes. The transformants were streaked on M9 agar plates in the pattern shown on the plate diagram containing the carbon source shown in either the presence or absence of biotin (bio). All plates were incubated at 37°C except those expressing <i>P. marinus bioK</i> which were incubated at 25°C. To prevent cross-feeding plates divided into three zones by plastic walls were used. <b>Panel A.</b> Arabinose as carbon source, STL24 <i>ΔbioH</i> transformed with pBAD322 carrying no insert (lower left third), expressing <i>bioG</i> or <i>bioK</i> (top third of each plate) and the wild type strain transformed with pBAD322 (lower right of each plate). <b>Panel B.</b> The inoculation pattern was the same as Panel A and glycerol was the carbon source in place of arabinose. <b>Panel C.</b> The streaking pattern is given by the plate diagram. Arabinose was the carbon source and the test strain was <i>E. coli</i> strain STL25 (<i>ΔbioCΔbioH</i>) transformed with pBAD322 carrying no insert (lower left third), <i>bioGC</i> (top third) or the wild type strain transformed with the vector pBAD322 (lower right third).</p

Topics: Biochemistry, Microbiology, Genetics, Biotechnology, Evolutionary Biology, genes, replaces
Year: 2013
DOI identifier: 10.1371/journal.pone.0049440.g004
OAI identifier: oai:figshare.com:article/215651
Provided by: FigShare
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