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Data from lower-titer injections. A.

By Joanne Clark (243279), Jessica M. Silvaggi (300062), Tomas Kiselak (300063), Kangni Zheng (243283), Elizabeth L. Clore (243281), Ying Dai (296727), Caroline E. Bass (300064) and David K. Simon (243285)

Abstract

<p>SYBR-green PCR analysis of Pgc-1α and Pgc-1α target gene mRNA levels after saline treatment in mice microinjected unilaterally into the right SN with AAV2/10-FHA-Pgc-1α or AAV2/10-mCherry. mRNA levels were normalized to 18S and fold-change gene expression was calculated relative to the contralateral SN using the ΔΔCt method. Data were analyzed using a paired <i>t</i>-test (n = 6). <b>B.</b> Striatal Th immunoreactivity data for the contralateral side (Cntrl) compared to the ipsilateral side microinjected in the SN with AAV2/10-FHA-PGC-1α. Ipsilateral and contralateral striatal Th immunoreactivity within each treatment group was analyzed using a paired t-test (n = 7). <b>C.</b> Mean levels of striatal DA per mg of protein in the ipsilateral and contralateral (cntrl) striatum of mice microinjected unilaterally with a lower titer of AAV2/10-FHA-PGC-1α. Data were analyzed by paired t-test (n = 5). <b>D.</b> DA turnover in each treatment group. Data were analyzed by paired t-test for within group comparisons and Student’s t-test for between group comparisons. <b>E.</b> SYBR-green PCR analysis of dopaminergic markers. Fold change in gene expression relative to the contralateral side was calculated as before. Data were analyzed using a one-sample t-test to determine if fold-change gene expression differed from 1 (n = 5). <b>F.</b> SYBR-green PCR analysis of <i>Pitx3</i> and <i>Nurr1</i> expression (n = 5). Fold change was calculated and data were analyzed as in E.</p

Topics: Genetics, Neuroscience, lower-titer
Year: 2013
DOI identifier: 10.1371/journal.pone.0048925.g006
OAI identifier: oai:figshare.com:article/218290
Provided by: FigShare
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