Cdc28 and Pho85 are static hubs with many dynamic interaction partners.

Abstract

<p>(<b>A</b>) Graph of expression ratios in the cell cycle, over time, showing non-periodic expression of the CDKs Cdc28 and Pho85 and the dynamic cyclins they interact with. Red dotted line indicates threshold used in the network analysis. Cyclins that act redundantly with other cyclins have not been shown (Cln2, Clb2,4,6). (<b>B</b>) Frames from the real-time rendering animation (threshold −0.2) from the network of Cdc28 and Pho85 and their interacting cyclins. At each time point, nodes (representing proteins) map gene expression data from the cell cycle to a green/black/red color gradient. Proteins and their interactions are hidden when their expression at any point in time falls below the threshold. The CDKs are present throughout the cell cycle, but their cyclins are not. Thus the cyclins do not have to compete with all the other cyclins to interact with a CDK but only those expressed at similar times.</p