<p>Representative gels illustrating the expression of H1 receptor, Vglut2, TRPC1, TRPC7 and TRPC5 transcripts in 10 (1–10) acutely dissociated MnPO neurons. The expected sizes of the PCR products are (in base pairs) 286, 393, 208, 245 and 243, respectively. Negative (−) control was amplified from a harvested cell without reverse-transcription, and positive control (+) was amplified using 1 ng of hypothalamic mRNA. Cells 4 and 6–10 were excited by histamine (50 µM). Cells 1–3 and 5 were not affected by histamine.</p
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