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Expression of genes associated to carbon fixation (Rubisco, <i>rbcA</i>) and synthesis of glycerol (glycerol 3-phosphate dehydrogenase, <i>GPD</i>).

By Luis P. Suescún-Bolívar (303333), Roberto Iglesias-Prieto (190638) and Patricia E. Thomé (303334)

Abstract

<p>RT-PCR from 1 µg RNA extracted from <i>Symbiodinium</i> type B17 (upper panel) or type A1 cultures (lower panel) exposed to control (left lanes) or osmotic stress conditions (right lanes) for 1 hr. The amplification products were separated in agarose gels photographed, scanned and quantified with the ImageJ program (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, <a href="http://imagej.nih.gov/ij/" target="_blank">http://imagej.nih.gov/ij/</a>, 1997–2011). Color was inverted in the scans for clearer visualization. Numbers represent relative intensity of bands normalized to transcript levels for <i>GAPDH</i> (glyceraldehyde 3-phosphate dehydrogenase, see methods).</p

Topics: Biochemistry, Cell Biology, Molecular Biology, Inorganic Chemistry, genes, carbon, fixation, synthesis, glycerol, 3-phosphate
Year: 2013
DOI identifier: 10.1371/journal.pone.0047182.g004
OAI identifier: oai:figshare.com:article/230847
Provided by: FigShare
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