<p>RT-PCR from 1 µg RNA extracted from <i>Symbiodinium</i> type B17 (upper panel) or type A1 cultures (lower panel) exposed to control (left lanes) or osmotic stress conditions (right lanes) for 1 hr. The amplification products were separated in agarose gels photographed, scanned and quantified with the ImageJ program (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, <a href="http://imagej.nih.gov/ij/" target="_blank">http://imagej.nih.gov/ij/</a>, 1997–2011). Color was inverted in the scans for clearer visualization. Numbers represent relative intensity of bands normalized to transcript levels for <i>GAPDH</i> (glyceraldehyde 3-phosphate dehydrogenase, see methods).</p
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