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TWEAK-induced apoptosis in PC-3 cells is dependent on caspase activation. A)

By Ana Belen Sanz (303062), Maria Dolores Sanchez-Niño (303063), Susana Carrasco (303064), Felix Manzarbeitia (303065), Olga Ruiz-Andres (303066), Rafael Selgas (260970), Marta Ruiz-Ortega (108389), Carmen Gonzalez-Enguita (303067), Jesus Egido (2652502) and Alberto Ortiz (108398)

Abstract

<p>Inhibition of caspases with pan-caspase inhibitor zVAD (20 µM) prevented apoptosis in PC-3 cells stimulated with TWEAK/TNFα/IFNγ for 24 hours. Apoptosis was assessed by flow cytometry of DNA content. Mean (±SD) of three experiments. *p<0.002 vs control; #p<0.001 vs TWEAK/TNFα/IFNγ alone. <b>B</b>) Incubation with TWEAK or TWEAK/TNFα/IFNγ resulted in the appearance of active caspase-3 fragments (arrows, representative Western blot), <b>C)</b> and this is barely observed in presence of 10% FBS. <b>D)</b> Active <b>c</b>aspase 3 immunofluorescence in PC-3 cells treated with TWEAK/TNFα/IFNγ. Confocal microscopy. Active caspase 3 in green and propidium iodide in red. (Magnification 320x). Pictures representative of three independent experiments.</p

Topics: Cell Biology, Physiology, Cancer, apoptosis, pc-3, cells, caspase
Year: 2013
DOI identifier: 10.1371/journal.pone.0047440.g010
OAI identifier: oai:figshare.com:article/230908
Provided by: FigShare
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