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Polysulfated glycosaminoglycan (PSGAG) inhibits complement activity in animals.

By Zhao-Hua Zhou (171194), Mohsen Rajabi (303010), Trina Chen (171196), Elena Karnaukhova (38273) and Steven Kozlowski (171203)

Abstract

<p>2E4 antibody-treated <i>E. coli BL21</i> bacteria were incubated with complement from pooled horse, donkey, pig or dog plasma, or incubated with the same pooled plasma pre-treated with 20 µg/ml of CSA, OSCS or PSGAG. Bacterial viability was determined by an SYTO9/PI staining bacterial viability kit and flow cytometry. Dead and live bacterial populations and percentages are indicated. Data shown are representative of three independent experiments.</p

Topics: Immunology, glycosaminoglycan, inhibits, complement
Year: 2013
DOI identifier: 10.1371/journal.pone.0047296.g007
OAI identifier: oai:figshare.com:article/231106
Provided by: FigShare
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