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The IBDV VP3 polypeptide counteracts VP2-induced cell responses when expressed from an eukaryotic plasmid expression vector. A. Induction of apoptosis.

By Idoia Busnadiego (135019), Ana M. Maestre (303493), Dolores Rodríguez (192288) and José F. Rodríguez (303494)

Abstract

<p>Cell monolayers were mock-transfected, transfected with pcDNA3, pcDNA-VP2, pcDNA-VP3, or cotransfected with pcDNA-VP2 and pcDNA-VP3. Apoptosis was measured using the Caspase-Glo 3/7 assay kit at 48 h. post-transfection. Each determination was carried in triplicate. Presented data correspond to the mean ± the standard deviation of three independent experiments. <b>B. PKR phosphorylation.</b> Total extracts from cells mock-transfected, transfected with pcDNA3, pcDNA-VP2, pcDNA-VP3, or cotransfected with pcDNA-VP2 and pcDNA-VP3 were harvested at 48 h. post-transfection. Extracts were subjected to SDS-PAGE, transferred to nitrocellulose, and immunoblotted with serum anti-VP2, -VP3, -PKR, or -pT451 PKR. The WB corresponding to PKR was used as protein loading control.</p

Topics: Biochemistry, Immunology, Virology, ibdv, vp3, polypeptide, counteracts, vp2-induced, responses, eukaryotic, plasmid, induction
Year: 2013
DOI identifier: 10.1371/journal.pone.0046768.g004
OAI identifier: oai:figshare.com:article/231507
Provided by: FigShare
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