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Anti-apoptotic effects of fibroblasts from the hHO-1-Tg pig.

By Hye-Jung Yeom (304014), Ok Jae Koo (304015), Jaeseok Yang (304016), Bumrae Cho (304017), Jong-Ik Hwang (135055), Sol Ji Park (304018), Sunghoon Hurh (304019), Hwajung Kim (304020), Eun Mi Lee (304021), Han Ro (304022), Jung Taek Kang (304023), Su Jin Kim (142497), Jae-Kyung Won (304024), Philip J. O'Connell (304025), Hyunil Kim (304026), Charles D. Surh (304027), Byeong-Chun Lee (304028) and Curie Ahn (304029)

Abstract

<p><b>A:</b> Cell viability was measured using the CCK-8. After induction with hTNF-α (25 ng/ml) and CHX (10 µg/ml) for 15 hours, the hHO-1 group had a lower percentage of viable cells. <b>B and C:</b> FACS analysis of hTNF-α and CHX-mediated apoptosis in fibroblasts. Apoptosis was analyzed using annexin V staining. The numbers indicate the proportion of apoptotic cells in the NPCCs after 15 hr incubation with hTNF-α and CHX. <b>D:</b> Activities of caspase 3/7 were measured in fibroblasts. Human HO-1 protected transgenic fibroblasts from apoptosis after 15 hr of incubation with hTNF-α and CHX. After Zn(II)PPIX treatment, Ad-hHO-1 increased activity of caspase 3/7. Each experiment was repeated three times. (* p<0.05, ** p<0.01, *** p<0.001, NT: no treatment).</p

Topics: Immunology, fibroblasts, hho-1-tg
Year: 2013
DOI identifier: 10.1371/journal.pone.0046646.g005
OAI identifier: oai:figshare.com:article/233946
Provided by: FigShare
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