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Expression of <i>Limks</i> in ocular tissues during eyelid closure.

By Dennis S. Rice (304216), Gwenn M. Hansen (304218), Feng Liu (72874), Mike J. Crist (304223), Matthew M. Newhouse (304224), David Potter (304225), Nianhua Xu (133512), Alejandro Abuin (304229), Peter J. Vogel (304230) and Brian P. Zambrowicz (304232)

Abstract

<p><i>In situ</i> hybridization in E15.5 specimens with radioactive riboprobes (as = antisense; s  =  sense) specific for either <i>Limk2</i> or <i>Limk1</i>. (A) High levels of <i>Limk2</i> are detected in the epithelium (ep) in both upper and lower eyelids in wild type mice (+/+). The epithelium in the cornea (c) and differentiated cells in the ganglion cell layer (gcl) of the retina express <i>Limk2</i>. The emerging epithelial sheets at the eyelid tips (arrows) exhibit more intense <i>Limk2</i> hybridization signals compared to adjacent epithelium. (B) Although the intensity of hybridization is decreased in the heterozygous (+/−) specimen, the distribution of <i>Limk2</i> is comparable to that in wild type. Higher levels of <i>Limk2</i> are present in the emerging epithelial sheets (arrows). (C) Homozygous mice, mounted and processed on the same slide as the specimens in A and B, show extremely low levels of hybridization. Three adjacent sections of another wild type E15.5 specimen hybridized with either the antisense <i>Limk2</i> (D), antisense <i>Limk1</i> (E) or sense <i>Limk1</i> (F). <i>Limk2</i> is expressed in the epithelium and gcl, whereas <i>Limk1</i> is detectable in the gcl. The intense signal in the retinal pigment epithelium (rpe in C and F) is an artifact of melanin that appears during dark field illumination and is present in all samples shown. Scale bar  = 100 μm.</p

Topics: Cell Biology, Neuroscience, ocular, tissues, eyelid
Year: 2013
DOI identifier: 10.1371/journal.pone.0047168.g005
OAI identifier: oai:figshare.com:article/234516
Provided by: FigShare
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