Expression of <i>Limks</i> in ocular tissues during eyelid closure.

Abstract

<p><i>In situ</i> hybridization in E15.5 specimens with radioactive riboprobes (as = antisense; s  =  sense) specific for either <i>Limk2</i> or <i>Limk1</i>. (A) High levels of <i>Limk2</i> are detected in the epithelium (ep) in both upper and lower eyelids in wild type mice (+/+). The epithelium in the cornea (c) and differentiated cells in the ganglion cell layer (gcl) of the retina express <i>Limk2</i>. The emerging epithelial sheets at the eyelid tips (arrows) exhibit more intense <i>Limk2</i> hybridization signals compared to adjacent epithelium. (B) Although the intensity of hybridization is decreased in the heterozygous (+/−) specimen, the distribution of <i>Limk2</i> is comparable to that in wild type. Higher levels of <i>Limk2</i> are present in the emerging epithelial sheets (arrows). (C) Homozygous mice, mounted and processed on the same slide as the specimens in A and B, show extremely low levels of hybridization. Three adjacent sections of another wild type E15.5 specimen hybridized with either the antisense <i>Limk2</i> (D), antisense <i>Limk1</i> (E) or sense <i>Limk1</i> (F). <i>Limk2</i> is expressed in the epithelium and gcl, whereas <i>Limk1</i> is detectable in the gcl. The intense signal in the retinal pigment epithelium (rpe in C and F) is an artifact of melanin that appears during dark field illumination and is present in all samples shown. Scale bar  = 100 μm.</p

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