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FoxO3a regulates Puma induction in potassium deprived CGNs.

By Kristin K. Ambacher (304535), Kristen B. Pitzul (304536), Meera Karajgikar (304537), Alison Hamilton (304538), Stephen S. Ferguson (304539) and Sean P. Cregan (304540)

Abstract

<p><b>A,</b> CGNs were maintained in high potassium (K25) or subjected to potassium withdrawal (K5). Protein extracts were collected at 8 hours and Phospho(Thr32)-FoxO3a and total FoxO3a levels were analyzed by western blot. <b>B,</b> CGNs were transduced with lentivirus (1 MOI) expressing shRNA directed against FoxO3a or a non-targeting control shRNA and FoxO3a protein levels were analyzed by western blot at 7 days post-infection. <b>C,</b> Puma mRNA levels were measured by qRT-PCR following 8 hours of potassium deprivation in CGNs transduced with FoxO3a-shRNA or non-targeting control shRNA (n = 3; *p<0.05). <b>D,</b> Phospho(Thr32)-FoxO3a and total FoxO3a protein was analyzed by western blot in CGNs maintained in high potassium (K25) or subjected to potassium deprivation for 8 hours in the presence or absence of either IGF-1 (200 nM), SP600125 (10 µM) or SB415286 (30 µM).</p

Topics: Cell Biology, Molecular Biology, Neuroscience, regulates, puma, induction, potassium, deprived
Year: 2013
DOI identifier: 10.1371/journal.pone.0046885.g010
OAI identifier: oai:figshare.com:article/236404
Provided by: FigShare
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