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PGE2 directly involved in inducing Treg migration.

By John Karavitis (305060), Laura M. Hix (217695), Yihui H. Shi (217696), Rachael F. Schultz (305061), Khashayarsha Khazaie (52401) and Ming Zhang (9736)


<p>CD4+ CD25+ Tregs were enriched by magnetic column (MACS) from naïve thymus and lymph nodes (A). Treg migration was measured using media alone, cell-free supernatant collected from TM40D, TM40D-COX2, or TM40D-MB cells (B). Additionally, the role of PGE2 was assessed by anti-PGE2 blocking antibody added to TM40D-COX2 media. Purified GFP-FoxP3+ cells were isolated from spleen following CD4+ T cell enrichment (C) and analyzed for expression of the PGE2 receptors EP1, EP2, EP3, or EP4 via RT-PCR with GAPDH as a control (D). #p<0.05, *p<0.01 compared to Media alone, N = 3 samples per group.</p

Topics: Immunology, Cancer, inducing, treg
Year: 2013
DOI identifier: 10.1371/journal.pone.0046342.g004
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Provided by: FigShare
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