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PGE2 directly involved in inducing Treg migration.

By John Karavitis (305060), Laura M. Hix (217695), Yihui H. Shi (217696), Rachael F. Schultz (305061), Khashayarsha Khazaie (52401) and Ming Zhang (9736)

Abstract

<p>CD4+ CD25+ Tregs were enriched by magnetic column (MACS) from naïve thymus and lymph nodes (A). Treg migration was measured using media alone, cell-free supernatant collected from TM40D, TM40D-COX2, or TM40D-MB cells (B). Additionally, the role of PGE2 was assessed by anti-PGE2 blocking antibody added to TM40D-COX2 media. Purified GFP-FoxP3+ cells were isolated from spleen following CD4+ T cell enrichment (C) and analyzed for expression of the PGE2 receptors EP1, EP2, EP3, or EP4 via RT-PCR with GAPDH as a control (D). #p<0.05, *p<0.01 compared to Media alone, N = 3 samples per group.</p

Topics: Immunology, Cancer, inducing, treg
Year: 2013
DOI identifier: 10.1371/journal.pone.0046342.g004
OAI identifier: oai:figshare.com:article/237992
Provided by: FigShare
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