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<i>HpaI</i> plasmid profiles of <i>bla</i><sub>CTX-M-94</sub>-, <i>bla</i><sub>CMY-2</sub>- and <i>bla</i><sub>CTX-M-100</sub>-harbouring plasmids.

By Jascha Vervoort (305513), Anna Baraniak (305515), Muriel Gazin (305517), Julia Sabirova (305519), Christine Lammens (305521), Meital Kazma (305522), Anna Grabowska (305523), Radosław Izdebski (305525), Yehuda Carmeli (224072), Samir Kumar-Singh (305527), Marek Gniadkowski (305528), Herman Goossens (305529) and Surbhi Malhotra-Kumar (305530)

Abstract

<p>A 0.7% agarose gel was loaded with <i>HpaI</i> restricted plasmid DNA purified from three recombinant <i>E. coli</i> TOP10 strains containing the (A) <i>bla</i><sub>CTX-M-94</sub>-, (B) <i>bla</i><sub>CMY-2</sub>- and (C) <i>bla</i><sub>CTX-M-100</sub>-harbouring plasmids, respectively, and stained with GelRed. Relative mobility calculations, based on known band sizes of the 1 Kb DNA Extension Ladder (M), estimated total plasmid sizes to be (A) 93 kb, (B) 98 kb and (C) 130 kb.</p

Topics: Biochemistry, Microbiology, Genetics, Infectious Diseases, plasmid, profiles
Year: 2013
DOI identifier: 10.1371/journal.pone.0046329.g003
OAI identifier: oai:figshare.com:article/239678
Provided by: FigShare
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