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Binding of radioisotopes of cesium with <i>P.</i> sp <i>binos.</i> a

By Hiroki Shimura (307990), Katsuhiko Itoh (307991), Atsushi Sugiyama (307992), Sayaka Ichijo (307993), Masashi Ichijo (307994), Fumihiko Furuya (307995), Yuji Nakamura (307996), Ken Kitahara (307997), Kazuhiko Kobayashi (307998), Yasuhiro Yukawa (308000) and Tetsuro Kobayashi (308002)

Abstract

<p>, 10, 30, and 100 mg/ml of wet algal cells were incubated with 1.92 kBq of <sup>137</sup>CsCl at room temperature for 10 min. Radioactivity accumulated in algal cells were measured, and data are shown as % of total radioactivity (mean ± SD). *, <i>P</i><0.05, **, <i>P</i><0.01 (n = 4). <b>b</b>, After 10 min incubation, the radioactivities of pellets and supernatant were measured and the bound/free ratios were calculated (n = 4). <b>c</b>, Ten mg/ml of wet algal cells were incubated with nonradioactive CsCl (solid circles) or NaCl (open circles) and 1.92 kBq of <sup>137</sup>CsCl at room temperature for 10 min. Radioactivity accumulated in algal cells were measured, and data are shown as % of control uptake without nonradioactive CsCl. Non-linear curve fitting to the model of two binding sites with high and low affinities was performed using the CsCl and NaCl competition data (n = 4).</p

Topics: Biophysics, Biotechnology, Plant Biology, radioisotopes, cesium, sp
Year: 2013
DOI identifier: 10.1371/journal.pone.0044200.g004
OAI identifier: oai:figshare.com:article/249134
Provided by: FigShare
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