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CYP3A4 gene expression regulation by calcitriol and astemizole in the hepatoma cell line HuH-7.

By Janice García-Quiroz (308054), Rocío García-Becerra (308055), David Barrera (41022), Nancy Santos (308056), Euclides Avila (41023), David Ordaz-Rosado (308057), Mariana Rivas-Suárez (308059), Ali Halhali (41027), Pamela Rodríguez (308061), Armando Gamboa-Domínguez (308063), Heriberto Medina-Franco (308065), Javier Camacho (308067), Fernando Larrea (41028) and Lorenza Díaz (41030)


<p>Cells were incubated in the presence of increasing concentrations of calcitriol (panel A) or astemizole (panel B) during 24 hours. Afterwards RNA was extracted for real time PCR analysis. Combined effect of both astemizole (3 µM) and calcitriol (10 nM) is shown in panel C. Results were normalized against GAPDH mRNA expression and in all cases vehicle values were set to one. In the box below the graphic: C = Calcitriol, A = Astemizole. N = 3, *<i>P</i><0.05 vs control. **<i>P</i><0.05 vs each compound alone.</p

Topics: Biochemistry, Pharmacology, Biotechnology, Cancer, calcitriol, astemizole, hepatoma
Year: 2013
DOI identifier: 10.1371/journal.pone.0045063.g007
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Provided by: FigShare
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