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PI3-K is required for resveratrol-inhibited the phosphorylation of AMPK and the expression of the SIRT1 protein induced by LPS in RAW 264.7 macrophage cells.

By Yi Zong (309128), Lin Sun (105539), Bin Liu (5899), Yi-Shu Deng (309129), Dong Zhan (309130), Yuan-Li Chen (309131), Ying He (125930), Jing Liu (38537), Zong-Ji Zhang (309132), Jun Sun (48981) and Di Lu (216447)

Abstract

<p>RAW 264.7 cells were pre-treated with resveratrol (5 µM) for 1 h in the absence or presence of wortmannin (1 µM), then exposed to LPS (5 µg/ml) for 40 min (Pane A) and 6 h (Panel B). Cell lysates (50 µg protein) were prepared and subjected to Western blot analysis by using antibodies specific for total AMPK, phosphorylated forms of AMPK (shown as phospho-AMPK), Sirt1 and β-actin as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044107#s4" target="_blank">methods</a>. The values shown are mean ± SEM of data from three independent experiments. <sup>#</sup>Significant compared with control alone, p<0.05. *Significant compared with LPS alone, p<0.05. <sup>Δ</sup>Significant compared with resveratrol + LPS, p<0.05.</p

Topics: Cell Biology, Chemistry, Immunology, resveratrol-inhibited, phosphorylation, ampk, sirt1, induced, lps, macrophage
Year: 2013
DOI identifier: 10.1371/journal.pone.0044107.g010
OAI identifier: oai:figshare.com:article/254067
Provided by: FigShare
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