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Effect of Tac in Th17 subpopulations of CD4+T lymphocytes from the peripheral blood of early-post transplant recipients.

By Byung Ha Chung (314449), Kyoung Woon Kim (314451), Bo-Mi Kim (314453), Shang Guo Piao (314455), Sun Woo Lim (314458), Bum Soon Choi (314460), Cheol Whee Park (314462), Yong-Soo Kim (42082), Mi-La Cho (46882) and Chul Woo Yang (277266)


<p>We used flow cytometry to examine how Tacrolimus regulates <i>in vitro</i> Th17 subpopulations of CD4+ T lymphocytes in Th17-polarizing condition. PBMC from renal transplant recipients were preincubated for 1 h in the presence of Tacrolimus and stimulated with Th17-polarizing condition for 48 h. Anti-CD3 (1 µg/ml), anti-CD28 (1 µg/ml), IL-1b (20 ng/ml), IL-6 (20 ng/ml) and IL-23 (20 ng/ml) were added to stimulate the differentiation of Th17 cells. Neutralizing antibodies to IFN-gamma (2 µg/ml) and IL-4 (2 µg/ml) were added in some experiments (R&D Systems). Flow cytometry of intracellular Th17 in CD4+ T cells stimulated in the presence of Th17-polarizing condition, assessed after 48 h and then stimulated for 4 h with PMA and ionomycin in the presence of GolgiStop. The data are representative of three independent experiments. The values are expressed as the mean ± SEM.</p

Topics: Immunology, Marine Biology, tac, th17, subpopulations, lymphocytes, peripheral, early-post, transplant
Year: 2013
DOI identifier: 10.1371/journal.pone.0042011.g007
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Provided by: FigShare
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