Tissue binding specificity of FC and mFC:LS for human tracheal and alveolar sections.

Abstract

<p><b>A</b>, Extensive staining of apical surface of human tracheal epithelia for the mFC: LS (green) against propidium iodide staining (in red) is observed. Bright staining of what appears to be goblet cells (inset at 20x magnification; indicated by <i>white arrow</i>) by this mutant HA resembles a similar pattern that was previously observed with 1918 H1N1 and 1958 H2N2 HAs. <b>B</b>, Shows minimal to no staining of apical surface of tracheal section by FC consistent with its low binding to human receptors on glycan array. <b>C </b><b>and </b><b>D</b> respectively show intense staining of alveolar section by mFC:LS and FC consistent with their high affinity binding to avian receptors.</p