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Respiratory LPS exposure increases vaccine-induced T cells in airway lumen (BAL) and lung interstitium at 14 days.

By Xuerong Chen (315045), Fangming Xiu (315046), Carly N. Horvath (315047), Daniela Damjanovic (315048), Niroshan Thanthrige-Don (315049), Mangalakumari Jeyanathan (315050) and Zhou Xing (95675)

Abstract

<p>(A) Experimental schema; At 14 days post-LPS or -PBS, mice were sacrificed and BAL and lung cells were isolated, and Ag85A tetramer (Tet)-specific (B) and IFN-γ-secreting (C) CD8<sup>+</sup> T cells in BAL or lung interstitium were determined by immunostaining, intracellular cytokine staining and FACS. Representative dotplots are shown with the average frequency ± SEM from three animals/group. The data in graphs are expressed as mean value ± SEM of three animals per group, representative of three independent experiments.</p

Topics: Medicine, Immunology, Infectious Diseases, Virology, lps, increases, vaccine-induced, cells, airway, lumen, interstitium, 14
Year: 2013
DOI identifier: 10.1371/journal.pone.0041666.g002
OAI identifier: oai:figshare.com:article/276068
Provided by: FigShare
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