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Binding of Alexa633-labeled DNP-HSA to TNP-specific Ig-fLCs bound to mast cells.

By Marco Thio (315020), Tom Groot Kormelink (315021), Marcel J. Fischer (315022), Bart R. Blokhuis (315023), Frans P. Nijkamp (315024) and Frank A. Redegeld (315025)

Abstract

<p>Murine bone marrow-derived mast cells (BMMC) were incubated with TNP-specific Ig-fLC (6 µg) or IgE (1 µg). After incubation, Alexa633-labeled DNP-HSA (300 ng) was added. Administration of Alexa633-labeled DNP-HSA resulted in increased binding to BMMCs sensitized with TNP-specific Ig-fLC (B, middle) or IgE (B, right) as compared to non-sensitized BMMC (B, left panel). To evaluate whether the binding of A633-DNP-HSA was specific, a 30-fold excess of unlabeled DNP-HSA (10 µg) was administrated simultaneously with the Alexa633 labeled-DNP-HSA. This resulted in a detectable loss in binding to Ig-fLC- and IgE-sensitized BMMCs (C). Panel A depicts untreated BMMC. Results are representative for 3 independent experiments.</p

Topics: Biochemistry, Physiology, Immunology, Developmental Biology, alexa633-labeled, dnp-hsa, tnp-specific, ig-flcs, bound, mast
Year: 2013
DOI identifier: 10.1371/journal.pone.0040986.g004
OAI identifier: oai:figshare.com:article/276217
Provided by: FigShare
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