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The effect of bicarbonate on secretagogue stimulated zymogen activation is decreased by PKA inhibition using H89.

By Thomas R. Kolodecik (297507), Christine A. Shugrue (314805), Edwin C. Thrower (314808), Lonny R. Levin (53582), Jochen Buck (53581) and Fred S. Gorelick (314811)


<p>Acini were treated with or without the PKA inhibitor H89 [10 µM] for 30 min prior to changing media and replacing it with either bicarbonate [25 mM] at a constant flow of air/CO<sub>2</sub> (95/5%) or without the addition of bicarbonate under room air conditions. H89 was added back to the appropriate wells. Secretagogue (CER 100 nM or CARB 1 mM) was added immediately after media change to the appropriate wells and acini incubated for 1 hour. Acini were collected and assayed for trypsinogen activation (A,D), chymotrypsinogen activation (B,E) and amylase secretion (C,F). #p<0.05 vs. corresponding secretagogue alone, *p<0.05 vs. corresponding secretagogue/bicarbonate treatment (n≥5).</p

Topics: Biochemistry, Molecular Biology, Chemistry, bicarbonate, secretagogue, stimulated, zymogen, activation, pka, inhibition
Year: 2013
DOI identifier: 10.1371/journal.pone.0041320.g009
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Provided by: FigShare
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